A researcher studying metastatic breast cancer cells measures gene expression at multiple levels. She obtains tumor tissue samples and determines messenger RNA levels of HER2 receptor, then quantifies the corresponding HER2 protein expression in identical samples. Previous Western blot analysis showed no protein degradation. Which pair of laboratory techniques should she use sequentially for these two measurements to assess transcriptional and translational gene expression, respectively?
- A)Western blot then Southern blot
- B)Flow cytometry then Western blot
- C)Northern blot then Western blotGABARITO
- D)Northern blot then ELISA only for DNA
- E)Southern blot then Western blot
Explicação
Northern blot detects specific RNA transcripts, whereas Western blot detects proteins. If the investigator wants to compare mRNA abundance with protein abundance, this is the classic pair to use. The question is testing the standard blot associations that rema... Ver explicação completa e trilha adaptativa →