A 52 year old woman with metastatic renal cell carcinoma enrolls in a trial of a drug that irreversibly binds an allosteric site on a signaling enzyme and reduces its catalytic activity. In vitro testing shows that adding more substrate does not restore the maximal reaction rate. Which of the following best describes the kinetic effect of this drug?
- A)Decreased Km with increased Vmax
- B)Decreased Vmax with unchanged KmGABARITO
- C)Increased Vmax with unchanged Km
- D)Decreased Km only
- E)Increased Km only
Explicação
Noncompetitive inhibition lowers Vmax because it decreases the amount of functional enzyme available, and the effect cannot be overcome by increasing substrate concentration. Km is unchanged in the classic pure form because substrate binding affinity is not al... Ver explicação completa e trilha adaptativa →