A 26-year-old woman presents with fever (38.9°C), myalgias, and nonproductive cough during a novel RNA respiratory virus outbreak. Vital signs show HR 102 bpm, RR 20/min, BP 118/76 mmHg, and SpO2 98% on room air. Nasopharyngeal swab shows positive rapid antigen test. However, confirmatory testing with higher sensitivity is needed. Which laboratory technique most appropriately identifies this novel RNA virus from the clinical specimen?
- A)Fluorescence in situ hybridization
- B)Western blot
- C)Southern blot
- D)Reverse transcription polymerase chain reactionGABARITO
- E)Flow cytometry
Explicação
Reverse transcription PCR converts viral RNA into complementary DNA and then amplifies it, making it ideal for rapid detection of RNA viruses. The respiratory outbreak setting and need to test a swab sample strongly favor this method. It is sensitive and highl... Ver explicação completa e trilha adaptativa →