DNA, extracted from a patient, is to be tested by PCR and DNA sequencing to ascertain the presence of a particular mutation. The DNA sequence to be amplified by PCR is shown below. The dashed line indicates the internal sequence where the potential mutation is located. Which answer choice represents the pair of primers that should be used in the PCR? (Sequence: TCCTGAGC--------------AAATGTGT)

1. A)GCTCAGGA and AAATGTGT
2. B)TCCTGAGC and TTTACACA
3. C)AGGACTCG and TTTACACA
4. D)GCTCAGGA and ACACATTT
5. E)TCCTGAGC and ACACATTTGABARITO