A family has a history of genetic disease with an autosomal dominant pattern of inheritance. DNA from the grandfather (who is heterozygous for the disease) is cloned and used to construct a restriction map of the gene region involved in the disease. He has a restriction site polymorphism at EcoHV site 2 (HV-2), which is useful as a marker. His abnormal chromosome lacks the HV-2 site. DNA from his affected grandson is digested with EcoHV, Southern blotted, and probed with 32P-cDNA complementary to exon 2. Which of the following options most likely represents the restriction fragment length polymorphism (RFLP) pattern obtained from the affected grandson?

1. A)BGABARITO
2. B)D
3. C)C
4. D)E
5. E)A