A scientist exposes a culture of a bacterium with minimal nutritional requirements to a chemical mutagen, then streaks out the bacteria on plates filled with a complex medium supplemented with all necessary nutrients. When the bacteria begin growing, she isolates individual colonies and tests each one for the ability to grow on a minimal medium. Cells derived from colonies unable to grow on a minimal medium are considered mutants, and their genome is sequenced. In one case, she finds that a 2-nucleotide segment of DNA has been deleted. This deletion would most likely give rise to which of the following?

1. A)Conservative mutation
2. B)Missense mutation
3. C)Nonsense mutation
4. D)Silent mutation
5. E)Frameshift mutationGABARITO